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Image Search Results


chvD sequence matches among types/reference strains

Journal: Infection and Drug Resistance

Article Title: The Homologous Gene of Chromosomal Virulence D ( chvD ) Presents High Resolution as a Novel Biomarker in Mycobacterium Species Identification

doi: 10.2147/IDR.S422191

Figure Lengend Snippet: chvD sequence matches among types/reference strains

Article Snippet: M. porcinum , ATCC 33776 , OP709746 # , RGM , M. peregrinum , 95.55% , M. fortuitum , 95.17%.

Techniques: Sequencing

Comparison of mycobacteria identified by 16S rRNA/ITS/ hsp65 / rpoB gene sequencing and chvD sequencing among the 179 clinical isolates a

Journal: Infection and Drug Resistance

Article Title: The Homologous Gene of Chromosomal Virulence D ( chvD ) Presents High Resolution as a Novel Biomarker in Mycobacterium Species Identification

doi: 10.2147/IDR.S422191

Figure Lengend Snippet: Comparison of mycobacteria identified by 16S rRNA/ITS/ hsp65 / rpoB gene sequencing and chvD sequencing among the 179 clinical isolates a

Article Snippet: M. porcinum , ATCC 33776 , OP709746 # , RGM , M. peregrinum , 95.55% , M. fortuitum , 95.17%.

Techniques: Comparison, Sequencing

DDX52 is overexpressed in human prostate cancer (PCa) and is associated with poor outcomes. a DDX52 expression in normal prostate tissues and PCa tissues was examined using immunohistochemistry (IHC) (Mann–Whitney test, error bars represent standard deviations; ***p < 0.001). b Representative photographs of normal prostate tissues and PCa tissues (scale bar: 100 μm). c , d DDX52 expression in the indicated human prostate tissues (data derived from the Gene Expression Omnibus datasets GSE6919 and GSE35988) ( t -test; *p < 0.05; **p < 0.01). e Kaplan–Meier plot revealing differences in the probability of survival between 25% higher DDX52 expression and 25% lower DDX52 expression in cohorts of advanced PCa patients

Journal: Cancer Cell International

Article Title: DDX52 knockdown inhibits the growth of prostate cancer cells by regulating c-Myc signaling

doi: 10.1186/s12935-021-02128-y

Figure Lengend Snippet: DDX52 is overexpressed in human prostate cancer (PCa) and is associated with poor outcomes. a DDX52 expression in normal prostate tissues and PCa tissues was examined using immunohistochemistry (IHC) (Mann–Whitney test, error bars represent standard deviations; ***p < 0.001). b Representative photographs of normal prostate tissues and PCa tissues (scale bar: 100 μm). c , d DDX52 expression in the indicated human prostate tissues (data derived from the Gene Expression Omnibus datasets GSE6919 and GSE35988) ( t -test; *p < 0.05; **p < 0.01). e Kaplan–Meier plot revealing differences in the probability of survival between 25% higher DDX52 expression and 25% lower DDX52 expression in cohorts of advanced PCa patients

Article Snippet: Antibodies against the following were used: DDX52 (NBP2-33776; Novus Biological, Centennial, CO, USA; western blot [WB]: 1:2,000, IHC: 1:500), c-Myc (ab32072; Abcam Cambridge, MA, USA; WB: 1:1,000, IHC: 1:100), Ki67 (sc-15,402; Santa Cruz Biotechnology, Santa Cruz, CA, USA; IHC: 1:200), AR (sc-816; Santa Cruz Biotechnology, Santa Cruz, CA, USA; WB: 1:1000), GAPDH (sc-365,062; Santa Cruz Biotechnology, Santa Cruz, CA, USA; IHC: 1:200), and vinculin (66305–1-Ig; Proteintech, Rosemont, IL, USA; WB: 1:1,000).

Techniques: Expressing, Immunohistochemistry, MANN-WHITNEY, Derivative Assay, Gene Expression

DDX52 knockdown impairs PCa cell proliferation in vitro. 22RV1 and PC3 cells were infected with lentivirus containing short hairpin RNA (shRNA) against DDX52 or the non-target control (shCON). a The efficiency of knockdown was verified using western blotting. Vinculin was used as the control to measure the loading quantities of the samples for western blotting. b , c 22RV1 and PC3 cells expressing shRNA targeting DDX52 or a control sequence were cultured in 96-well plates for 5 days. The growth rate in each group was measured using the Cell Counting Kit-8 assay ( t -test, error bars represent 95% CIs; ***p < 0.001). d The viability of 22RV1 and PC3 cells expressing shRNA or shCON was determined using a colony formation assay. e Histogram showing the number of colonies in each group ( t -test, error bars represent 95% CIs; ***p < 0.001)

Journal: Cancer Cell International

Article Title: DDX52 knockdown inhibits the growth of prostate cancer cells by regulating c-Myc signaling

doi: 10.1186/s12935-021-02128-y

Figure Lengend Snippet: DDX52 knockdown impairs PCa cell proliferation in vitro. 22RV1 and PC3 cells were infected with lentivirus containing short hairpin RNA (shRNA) against DDX52 or the non-target control (shCON). a The efficiency of knockdown was verified using western blotting. Vinculin was used as the control to measure the loading quantities of the samples for western blotting. b , c 22RV1 and PC3 cells expressing shRNA targeting DDX52 or a control sequence were cultured in 96-well plates for 5 days. The growth rate in each group was measured using the Cell Counting Kit-8 assay ( t -test, error bars represent 95% CIs; ***p < 0.001). d The viability of 22RV1 and PC3 cells expressing shRNA or shCON was determined using a colony formation assay. e Histogram showing the number of colonies in each group ( t -test, error bars represent 95% CIs; ***p < 0.001)

Article Snippet: Antibodies against the following were used: DDX52 (NBP2-33776; Novus Biological, Centennial, CO, USA; western blot [WB]: 1:2,000, IHC: 1:500), c-Myc (ab32072; Abcam Cambridge, MA, USA; WB: 1:1,000, IHC: 1:100), Ki67 (sc-15,402; Santa Cruz Biotechnology, Santa Cruz, CA, USA; IHC: 1:200), AR (sc-816; Santa Cruz Biotechnology, Santa Cruz, CA, USA; WB: 1:1000), GAPDH (sc-365,062; Santa Cruz Biotechnology, Santa Cruz, CA, USA; IHC: 1:200), and vinculin (66305–1-Ig; Proteintech, Rosemont, IL, USA; WB: 1:1,000).

Techniques: Knockdown, In Vitro, Infection, shRNA, Control, Western Blot, Expressing, Sequencing, Cell Culture, Cell Counting, Colony Assay

DDX52 knockdown inhibits PCa xenograft growth. a The mice were killed at 6 weeks after implanting 22RV1 cells, and the volumes of the xenograft tumors are shown. b Weights of the xenograft tumors in the control and treated groups (Mann–Whitney test, n = 7, error bars represent standard deviations; ***p < 0.001). c Kaplan–Meier plot showing the time of xenograft genesis after implantation (log-rank test; ***p < 0.001). d Examples of tumor xenografts that underwent hematoxylin and eosin (HE) staining and DDX52 and Ki67 immunohistochemical staining (scale bar: 100 μm). e , f The positive cells in random areas in each xenograft section were counted. e DDX52 expression was measured according to the IHC score (Mann–Whitney test, error bars represent standard deviations; **p < 0.01). f Ki67 expression was measured according to the number of positively stained cells (Mann–Whitney test, error bars represent standard deviations; **p < 0.01)

Journal: Cancer Cell International

Article Title: DDX52 knockdown inhibits the growth of prostate cancer cells by regulating c-Myc signaling

doi: 10.1186/s12935-021-02128-y

Figure Lengend Snippet: DDX52 knockdown inhibits PCa xenograft growth. a The mice were killed at 6 weeks after implanting 22RV1 cells, and the volumes of the xenograft tumors are shown. b Weights of the xenograft tumors in the control and treated groups (Mann–Whitney test, n = 7, error bars represent standard deviations; ***p < 0.001). c Kaplan–Meier plot showing the time of xenograft genesis after implantation (log-rank test; ***p < 0.001). d Examples of tumor xenografts that underwent hematoxylin and eosin (HE) staining and DDX52 and Ki67 immunohistochemical staining (scale bar: 100 μm). e , f The positive cells in random areas in each xenograft section were counted. e DDX52 expression was measured according to the IHC score (Mann–Whitney test, error bars represent standard deviations; **p < 0.01). f Ki67 expression was measured according to the number of positively stained cells (Mann–Whitney test, error bars represent standard deviations; **p < 0.01)

Article Snippet: Antibodies against the following were used: DDX52 (NBP2-33776; Novus Biological, Centennial, CO, USA; western blot [WB]: 1:2,000, IHC: 1:500), c-Myc (ab32072; Abcam Cambridge, MA, USA; WB: 1:1,000, IHC: 1:100), Ki67 (sc-15,402; Santa Cruz Biotechnology, Santa Cruz, CA, USA; IHC: 1:200), AR (sc-816; Santa Cruz Biotechnology, Santa Cruz, CA, USA; WB: 1:1000), GAPDH (sc-365,062; Santa Cruz Biotechnology, Santa Cruz, CA, USA; IHC: 1:200), and vinculin (66305–1-Ig; Proteintech, Rosemont, IL, USA; WB: 1:1,000).

Techniques: Knockdown, Control, MANN-WHITNEY, Staining, Immunohistochemical staining, Expressing

DDX52 affects the activation of c-Myc signaling in PCa. a , c Gene set enrichment analysis (GSEA) performed based on hallmark gene sets indicated that the c-Myc-upregulated gene set was the most significantly enriched in patients with high DDX52 expression in the indicated PCa cohorts. The results are shown in rank order according to the normalized enrichment score and the p-value. b , d The c-Myc-upregulated gene set was used to perform GSEA for the respective datasets based on the DDX52 transcription level. e , f GSEA of the expression profiles obtained from RNA sequencing of 22RV1 ( e ) or PC3 ( f ) cells with (shDDX52, right) or without (shCON, left) DDX52 knocked down based on the c-Myc-upregulated gene set.

Journal: Cancer Cell International

Article Title: DDX52 knockdown inhibits the growth of prostate cancer cells by regulating c-Myc signaling

doi: 10.1186/s12935-021-02128-y

Figure Lengend Snippet: DDX52 affects the activation of c-Myc signaling in PCa. a , c Gene set enrichment analysis (GSEA) performed based on hallmark gene sets indicated that the c-Myc-upregulated gene set was the most significantly enriched in patients with high DDX52 expression in the indicated PCa cohorts. The results are shown in rank order according to the normalized enrichment score and the p-value. b , d The c-Myc-upregulated gene set was used to perform GSEA for the respective datasets based on the DDX52 transcription level. e , f GSEA of the expression profiles obtained from RNA sequencing of 22RV1 ( e ) or PC3 ( f ) cells with (shDDX52, right) or without (shCON, left) DDX52 knocked down based on the c-Myc-upregulated gene set.

Article Snippet: Antibodies against the following were used: DDX52 (NBP2-33776; Novus Biological, Centennial, CO, USA; western blot [WB]: 1:2,000, IHC: 1:500), c-Myc (ab32072; Abcam Cambridge, MA, USA; WB: 1:1,000, IHC: 1:100), Ki67 (sc-15,402; Santa Cruz Biotechnology, Santa Cruz, CA, USA; IHC: 1:200), AR (sc-816; Santa Cruz Biotechnology, Santa Cruz, CA, USA; WB: 1:1000), GAPDH (sc-365,062; Santa Cruz Biotechnology, Santa Cruz, CA, USA; IHC: 1:200), and vinculin (66305–1-Ig; Proteintech, Rosemont, IL, USA; WB: 1:1,000).

Techniques: Activation Assay, Expressing, RNA Sequencing

c-Myc regulates DDX52 expression in PCa. a , b 22RV1 and PC3 cells were infected with lentiviruses carrying shRNA against DDX52, c-Myc or shCON, and gene expression was determined using western blotting. c Published PCa datasets were analyzed to assess the correlation between mRNA expression of DDX52 and c-Myc. f Representative photographs of PCa tissues stained with DDX52 and c-Myc (scale bar: 100 μm). e Correlation between the expression levels of DDX52 and c-Myc as determined using the IHC score (n = 86)

Journal: Cancer Cell International

Article Title: DDX52 knockdown inhibits the growth of prostate cancer cells by regulating c-Myc signaling

doi: 10.1186/s12935-021-02128-y

Figure Lengend Snippet: c-Myc regulates DDX52 expression in PCa. a , b 22RV1 and PC3 cells were infected with lentiviruses carrying shRNA against DDX52, c-Myc or shCON, and gene expression was determined using western blotting. c Published PCa datasets were analyzed to assess the correlation between mRNA expression of DDX52 and c-Myc. f Representative photographs of PCa tissues stained with DDX52 and c-Myc (scale bar: 100 μm). e Correlation between the expression levels of DDX52 and c-Myc as determined using the IHC score (n = 86)

Article Snippet: Antibodies against the following were used: DDX52 (NBP2-33776; Novus Biological, Centennial, CO, USA; western blot [WB]: 1:2,000, IHC: 1:500), c-Myc (ab32072; Abcam Cambridge, MA, USA; WB: 1:1,000, IHC: 1:100), Ki67 (sc-15,402; Santa Cruz Biotechnology, Santa Cruz, CA, USA; IHC: 1:200), AR (sc-816; Santa Cruz Biotechnology, Santa Cruz, CA, USA; WB: 1:1000), GAPDH (sc-365,062; Santa Cruz Biotechnology, Santa Cruz, CA, USA; IHC: 1:200), and vinculin (66305–1-Ig; Proteintech, Rosemont, IL, USA; WB: 1:1,000).

Techniques: Expressing, Infection, shRNA, Gene Expression, Western Blot, Staining

Reagents, cell lines, mice and oligonucleotides

Journal: The Journal of Neuroscience

Article Title: A Novel CCK Receptor GPR173 Mediates Potentiation of GABAergic Inhibition

doi: 10.1523/JNEUROSCI.2035-22.2023

Figure Lengend Snippet: Reagents, cell lines, mice and oligonucleotides

Article Snippet: Anti-GPR85 , Thermo Fisher Scientific , PA5-33776.

Techniques: Imaging, Stable Transfection, Expressing